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CRIMIC CR70400

Gene bru1

CG31762

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:12175004..12313438	33D2-33E1	FBti0226864	97165

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTGTTCGCTTGCAGCC	GAGAATATTTGAGTGCCGTCGAG

Images

Construct Design Sequences

sgRNA sequence	TTAAGCAGCCGAGATAGTTAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGGCCGCAGGACTTTTGACCCCGATGACCGTCCAGAATCTAGCGGCCATTGCGGCCATGACGCAGCCGAGTCTCTCGAATGCCGCCGCGGCAGCCGCCGCTGCCACATCGCCTGGAAGCGCCCAACTGACCAACACGGCTGCGCTGCTCTGTGAGTTACTCCTTCACATCCTTAAACAGCTATTTAAGCAGCCGAGATAG	TTAGGGGCAAAAGCACTTGAAGCGCCTCCATGGTTAACAATTCATCAGGGCTTACCGCATCCCATAATCTCGCGGCCTTATCTGGGCCATTAGTTGCCTCGGCGGCTAATTATCTAATTTTTTTCTCATTTCCGTATTGAAATTTTCCTTGTGCGTCACAGGGTTTTTCCACATCCACTTCTCTATTTTCTTGTTAGCCT

 

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