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CRIMIC CR70402

Gene SppL

CG17370

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:25881984..25888230	96F5-96F6	FBti0226866	97167

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGAAGAAGCGAAATGAATCGATG	ATGGGCAGCAGCAGAAAGG

Images

Construct Design Sequences

sgRNA sequence	GACTGCGTTACTGCATTGTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCACTCTGGACACGATGCATGCCCTTTGTTTGCCCCTCGGAGCTTCCATCTCATTGCTCATCATGTTCTTCTTTTTCGACTCAATGCAGCTGCTCTTCGCCGTCTGCACAGCGAGTAAGTGTGGTTAAATTATATATATTTCTACCCGTTTATCTGCTTATGGTTTCTTTCAAAAATATTTAATAAAAGGGGTCCACAC	AATGCAGTAACGCAGTCACAAGCTACTCTTCGAATCACATTCAAGCTTTTTAATTATAAATTTATGCGCTGATAAACTCATACTTCTTTTAATTTTCAACTCGAAAGCGATAAAAGCCATCAGAACCATTCAGCTTGCATACTTATTCAAGACATGACAAATTTGTTGCTTTATTAAATAATTTTAAATCTCTCTCCACA

 

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