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CRIMIC CR70403

Gene zyd

CG2893

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:23022898..23035550		FBti0226867	97168

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAGCTTTGGCTCACAAAG	CAAGAAGCTGTCACGCCC

Images

Construct Design Sequences

sgRNA sequence	AAAACATGGCTTGATTGGCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTCATTAGTCGGAAATATAATGTCAATTTGCTTTATTATAGAACAAACTATAGTTCTTATACTTTTTACGCAAGTTACTCATAGAATATATGGTATGATAAAATTCGATGAATACTATACTACGTAATAGGGGGGAACGAAAGGAAAACGCTACCCAATAAACTATATATACTGTCGAATCGGATCACTAGCAGCCAAGC	CAATCAAGCCATGTTTTACATTTTTTATTTCTCGATTTTTGTCACGCCCATGTTGATCGATATGCGGAAAATCCTTTTTGACCATACCCATTCTATCGCATGCATATTACCAAACCATTTATATAACAAATTTGGAAAAAAATTAATGAAAGAAAAGTAATGCTACAAGGAGTCTGTAGTACATTTTGATTTTTTGTTTA

 

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