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CRIMIC CR70405

Gene tbrd-3

CG30417

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:23326739..23327581	59D6	FBti0226922	97316

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ATATCTTCGATATCGACTATCGG
sgRNA2 sequence	ATCTGAAACTCAATTATATTTGG

 

left_200bp	right_200bp
CTTGCATAATTAACTGAATTTGTTTGTTGCCCCAAACCGCATTTGTGGATTGACTTTTCTGCTCGGATTGAGGTGGCAATCCTAAAAGTTTTCATAAGCTAAAAATATCATCGTACAGGGCAACCCGGAGTGTGAATATTGTCAGTTGTATTGCGTTCAAGTTGTCAAAACGATAGAAATTATAATAGAATTTTCCGATAG	ATAATTGAGTTTCAGATTGAAAAAGAGTTTTAATTTATTGTAATCTAGTCCTTTTTGCCCAAAAGCTTCTCCTGCTCCTTTCTCTTGTCGCGCATGGAGCGGTAGTTCCGGAAATCCCCATTCCACGTCTTGTTACGCATGATCTCCAGCGGATTGGAAGCTATGTAAGCGAAAAAGGTCTTCCGAGGAGGTCCTCCGGG

 

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