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CRIMIC CR70410

Gene da

CG5102

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10388105..10393228	31E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse

Images

Construct Design Sequences

sgRNA sequence	TTGCAGATCTATTGCTGAAATGG
sgRNA2 sequence	CTGTTCCATTACGTTTAACCTGG

 

left_200bp	right_200bp
ATAACCTGTTGCATTAAATAAATTTAAAGTAAATTAATTACACCTAGCTCACGGAATTATTATTATTTTTGTTCATATATCTTCAAATATATGATCTAGTCCTTAGCTTTCCTTTTTAATCGAACCATTCAAGTATGCGAATTACATGTGTAGACAGCATTAACTTATGTCCCCCCCGTTGTTTTGCAGATCTATTGCTG	ACCTGGGAAGATCTTTTTCGTTTTTTAGAATGTTTGATTTTAATAATTATTTGTTTTATTTTGTGCGATATCACCGTAGCCATATTTTTTATATACAATTACATGCATACATAACGACATAATTAAGTGAAAATAGTTTTATAATAAAAAAAAACACAACACATAGACTTACTTCTATATATTCCCGAGAGCTAAGGAAT

 

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