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CRIMIC CR70414

Gene Dip-eta

CG14010

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:5601723..5617946	25F1		98480

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAGGATCTGCACTTATGG	CGCTGTCATATTCACAATTGGAGAAC

Images

Construct Design Sequences

sgRNA sequence	ATCTATTTGTGAATGCCTTGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATGAGTCAACAATGTTATCCTCAGAGAGTAGAGGTGCCAGCTGAAGTAATAGGTAAGTCCCTACTTATTTTAAAGTCATTCAACAAATGGTAAATGTTAACTATTGATGTTTCTGATTGAAACATTTATCAGCTAAACTGATTTGATTACTTATGTATTGTGATTTAACGCTGACAGAAACTTCAAAACATCGCCGCAA	GGCATTCACAAATAGATATTTTGCGAGCTAGTTAAATATGTCATTATTTGACATTAATAACTCCATTGCCATTCGGCGTTTGACGATTTCCCGAACACATATCTAATAATATTCAGAATATGACATAAATGTTATGTTTTTATTTAGCTATCATCATATTCCATACACTGCATTATATTCCAATTATTTGCCAATCAAGC

 

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