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CRIMIC CR70419

Gene CG5946

CG5946

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:11816695..11822275	68E1	FBti0226869	97170

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTAATGGGTCTCGGACTTG	AAGCTGATTGCACTGACTGC

Images

Construct Design Sequences

sgRNA sequence	ATTGCGTTTGGTCTTCCGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTAAAGTTGGAAGTCTCAACCTAGTAAACCAAATTAGCAGCTGTTTTTTTTAAAGGGCGCCTTCGCTTTGCAATCACATATATCAGTTTTGAGTGCGAATAATCTGTGTGCGAGATGTGTTTTTTATTTTTGTGACTTTCTTATCACTGGCGGTAGTTTCCACCAGCCACAGTGACCTTTCATAGGACATCAGCCCATC	GGAAGACCAAACGCAATATCACTGGGCTAAAAATTAGCTCAACTAAATATCAGATTATTAATACAATACAATATACATGCTTACCATTTAAATGAAGTAAAATATTACTATATTAAAAGATATGATCATTTTTGATCAGGACCAATAGCAGAGTCAAACTGGACTTTTTTAATTTGGTTTAATATTTTAAATATGACAAA

 

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