CR70423

CRIMIC CR70423

Gene CG18473

CG18473

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:9363305..9364838	85D11-85D12	FBti0226923	97317

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTATCATTGCAATTAAGATCACCTGC	GAGACTTATCTCTAATCGGCAGTGG

Images

Construct Design Sequences

sgRNA sequence	CCACTGGTCTTCGTAGAGATCGG
sgRNA2 sequence	TCTTGTTGATAAGATTAGCGCGG

left_200bp	right_200bp
TTCTGATGCTTCATAATGAGAACTAATGGAATGATCAACAACTCAGCCCACTGCAATCTCTGATTGTGCGCAATAGAATATTTTTTAGATAAGACGTGGTCCGGGGTATCAAGCAAAAGCAACTGTAAACATATATCTGCGGTTGGGGAACTCCACAGTGAACCGTCTCTCGAGATCAGTTCTCCACTGGTCTTCGTAGA	GCGCGGTTCTTTTGGCAATAAAAAATGGGCGTGTCTTCGAACTCAAGCTAATCACTTTGTTTTTATTACAAAACACGAAAATGGGGGCTCGTACGATTTTTTTTAGATGCAATTAGAAAGTGATTAATAAAAACTTGGGCGACCACAAAAATAAGTCCTCTACCTTTGTTCTATATCAGTTGATTAAATATATGGATATA