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CRIMIC CR70425

Gene Psn

CG18803

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20432468..20435156	77C3	FBti0226924	97730

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCTTCGCAAATTAATCGAACATC	CAGAAGTACGGCTATCAGCTGC

Images

Construct Design Sequences

sgRNA sequence	GAACGAACGCACACCACTGATGG
sgRNA2 sequence	TATATAAACTTGAAAAGACAAGG

 

left_200bp	right_200bp
CTAGTATATGAGTCGCTTTAAAACAAAAGAAAGTTTTTACCAGCTACATTTCTTTGGTTTCCTTAACTGTGAGTAGTCCAACTCCTGCGAGTGTCAAGCTCGGTCCTAATCGGTGGTGCTCCCCAAGAAATCCCATCACACAACTACGGCTTCGCAGGGGGAGGCGTCCAGCGCTACGGAGGCGAACGAACGCACACCAC	ACAAGGACACATCAAGTGTCTTACAGTATCATAGTCTAACAAAGCTTTTTGTAATCCAATTCTTTATTTAACAAAATGCATAGTAACAACCTCGACTAATTATTGCTTGCGCTTAATGATCGTTTTGTCCACTATGTTGATCAGCCAGCCGGGTTCCAGGCCGTAGAATACTTGCCTTGGATCCTTCGCTGTGGTGAGCA

 

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