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CRIMIC CR70431

Gene sav

CG33193

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:23061304..23061304	94D10	FBti0226871	97172

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAACTAGGTAAACTGCCTCTTG	CGTAGATCGGGTACGAATCGC

Images

Construct Design Sequences

sgRNA sequence	ATTGTAAGGCGAGTAGAAACCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATATGAGCAGCTCTTGATTATACGAATAATCCGTAAAAACTACATATTGTCAAAAAGTGCTTAATATTATTATCACTTTTAAACAATGACAATGCACGAAATATTTTATTTTCGAAACATATATTGTTCAAAGATTTTGTATTTGATAACAGATTGCTTTATTTATTTACAATAAGAAAAATTGATGTACAAAACCGGTT	TCTACTCGCCTTACAATAATTAAAACAATAACACAATATATGATTTTCTGTACGAGGAATATAATGGAATATATATGATATATACAACATTTTTAAACACATTTTCTCTTCTGTTTCCACAGCTCTCCGATGCAGCAGCAACAACAACAACAACAACAGCAGCAGCAGCAGCTGCAGCACACACAACTGGCAGCTCACCT

 

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