| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_PM37_kG4 | 2R:15538175..15540014 | 52A10 | FBti0226926 | 97319 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | CGATCAACCCACCGAAGTGC | CGAGAGCAAGAACAAATTCGTGC |
 |
| sgRNA sequence | TTCGATCTGGATCCGGGTCTGGG |
| sgRNA2 sequence | TAAACAACATGCCTCCATGATGG |
| left_200bp | right_200bp |
|---|---|