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CRIMIC CR70434

Gene lap

CG2520

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:7186994..7200941	84D1-84D2	FBti0226872	97173

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCAACAACATTCAGATGCAACCAG	GTGCCAACAAGCTAAATAAAAGGG

Images

Construct Design Sequences

sgRNA sequence	ACGGGTGCACGTGTGTTTGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTTTTCTGAAAACGAAACCACTGATTACGGTCCACAAAGTCCCACAAACACAAACCACCACCCTCAAATTGCATCTGTGGCTCAGCTGCTGGTGTTTGTGGACTCCGTTCGTCATTGATTGGACTCCCCTCTCTTTCTACCAACACACTCTCTCTCTCTCTCTAATGTTACGTCACTTTCCCACGGGTGCACGTGTGTT	TGTAGGGGCTTTGAATGGCTTAAAACGCCTACGAAAGCAGGGATTGTGTTTGTCACGGTGTCATTGCTTGTTAAATTTATGCAAGAGTCTTCCCTAATTTGCTGGATTTGCTTACACGTTTGAATTTTGCAAATACTTCTAAAGAGCTCCATATCCTAACGACATTCATTCCCGTTGGAATCTGTTTTTTTGGCTAGTTT

 

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