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CRIMIC CR70437

Gene Trm1

CG6388

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12428028..12430021	33E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CTGGTGGTGGCAAAGTAGC	GCAAGCAGGAGGACGTATTGG

Images

Construct Design Sequences

sgRNA sequence	TGAGTTAAAAGAATACACGTGGG
sgRNA2 sequence	ACGCAAAATGGTATTCTTTCTGG

 

left_200bp	right_200bp
CCATTTTTATTCAACATCATTTTATGGGATGTATTATTACAGAGAATATACCCAAATTGTAAATTTTGAAACTAAATTAAACTTAAATATAATTCTGTAATGTTTATTTCGTATTTCATATAAAAAACAACAAATTTGTGTATATTTTTAGTTTTAAAGCTTAATAGCTAGTAGCTTACGTTTTTCAACACTGCCCCACG	TTCTGGGAAAGCAAACACAGATTTGTGGAACTAATAGCGGAAGAAGTTGCCGTCGGAGGTCTGGCGCGAGAATCCATTGTTCACCAGCTGGTTGTGGACACCGCTGCAAATGAGCTTTACGCTGTGCGGATGAATCTTTTCTTTACAACCGCCTGCAACAATATGATTAGATTCGAAGTGGGTGTTACTTAAAATGCGTC

 

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