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CRIMIC CR70440

Gene Mef2

CG1429

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R :9958858..9913496	46C4-46C7	FBti0226874	97175

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTGCTATGCATCCGAATATTGACC	CGGAAACGGAAGTGCGTTC

Images

Construct Design Sequences

sgRNA sequence	TTCTGGCTGAGGCTACGTCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTCAGTTATTTTTTAATATAGTTCTTTATAACTTTCACAAAACTATCAAAATTTAAAGGCAGGCGTTTAAAAGTAGTTTTGCCTCTTTAGCACGGATATAATTTTCCTTTGCCGTACCCATTTTTTAACTGCCAAGCGTGTGCCGTGTCATCGCAAGGTTTTCCCACCGACTTTTCGTTCCTTTCTGGCTGAGGCTACG	TCTTGGCCAAGCCATAAACGCGTCATCGTCGAGCAGTGAAAAACAAAAACAAAAAAAAAAAACAGAACAAGGAACAGTAACAACTTGTTTATGGTGCTGCCCCTTTGGCCTTGACTCGGCCATAATCGATTCATTACCAACTAGGCAGCTAGGAAACGAGCAAGGGCCTGGGCCACTTTCCCACTTGGCCGGGCCACTTT

 

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