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CRIMIC CR70443

Gene CG8243

CG8243

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:8922785..8925346	44F7-44F8	FBti0226875	97176

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCATGTGAAGCAGAGATAAGCTCC	CCATCTGCTGCAGCGAGATC

Images

Construct Design Sequences

sgRNA sequence	CACAGATGTGCGTCACCAAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGAAAGCAGGCCTCACAGACCGCAAGCATACCCCAAAATGAGCTCCTCGAATGCGGGACAGCGCACGAAGCTAATTCAGGAGAAGTGCCAGACCCTCCTGACGCAGATGCTCCGCGACGAGGACAACAAGTACTGCGTGGACTGCGATGCCAAGGGTATGTTAATGGGGTCCACTTCTCCGCCAAGAGATACGCCTCTT	GGTGACGCACATCTGTGTGCGTGCGTGCGTGGATATACACACCACACATGCAGCTGCACTGCGATATGAGCCTATGTGCTGTCACTGAATGCTACCTTTTATCCTTAGGTCCGCGCTGGGCCTCCTGGAACCTGGGCATGTTTCTCTGCATCCGCTGCGCCGGCATCCATCGCAATCTGGGCGTGCACATATCGCGCGTC

 

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