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CRIMIC CR70445

Gene tefu

CG6535

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:15232212..15242236	88E3-88E4	FBti0226929	97322

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGCGCTGAACGTAGCTTG	GTCTCGATAAGCGTTATTTTTACAACCG

Images

Construct Design Sequences

sgRNA sequence	ACATTACAACAGGTTATGCCGGG
sgRNA2 sequence	CAGAGCGGAAAACCCGTAAGTGG

 

left_200bp	right_200bp
TAAATCTTTCGTTATATAAGGTTATTCTAGTTCTCCATCGGCACTTATTTGACGCACACGGCTGCCCAGCCATTTAACAACAGTGCTGTTCTTATTGATCGTGGTATTTTTTGGCGCTCTGGCAGAGGTCACTCTAAGTACATTTTGAGAGATCATTTTATGTTTAGTTTAATAATTAATACAACATTACAACAGGTTAT	AAGTGGTATACATTATCTTGGCAAGTGAAGAATTTCCATCCAATATTTCGACATTTAAAATTTTCAAAGCTTATGGCATTCGTTTTTCAAAATGCAGGTTACATATATTTAATCTATGCCCGTTTTTTTCTGTGCATTACATGGTCTCTAAATTATTGGCCCCCAGCAAGTCTTAGGAATTAGTTGGCCTCTACTTTTTT

 

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