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CRIMIC CR70446

Gene GP150

CG5820

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:22317662..22329416	58D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GCTTTGGCTGATGATCCACACTTC	CTTCTGGATGCCCATGTCAAGTCT

Images

Construct Design Sequences

sgRNA sequence	TATTTGAATTTAAGGATGATGGG
sgRNA2 sequence	TAGAATCAGCAGTTCCGGGCCGG

 

left_200bp	right_200bp
CAATACTACCGTCGCCGCCGGGGAGAAGAACTCCACCGATACCGACGATAGCGAATACTATGACTACGATGATGATTACAACTATTTCGATGAGGAGACGGTGACGATTTCCCCCAGCCCCAAGAAGGCCAAGAGCAACCCCAAGCTGCAGCCCATTGTGGATGCCACCATCACGAAGTCCAAGGTATACTTCCCACATCATCCTTAAATTCAAATAAATACGTTTTCGTACTTTACA	GGCCGGTGGCCGCGGAAATCGAACGAGTTGCGCTGTTTTTCTAATTTATAAGTATATTGATAGTTGAAAAAGTGGAAAATCACATCAGACAATTGACTACACACAAGCACAAGAATGTAAGAAACAGGAGGAGGAGCAGTAGGTGGCAGTTAAAAGTATTAGCGAAAGTGCGAGGAAACAAAGCAACAACTAAAAAATAC

 

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