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CRIMIC CR70447

Gene CG31381

CG31381

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:24877045..24878827	96B2	FBti0226930	97323

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTATATTCCGGCAGAATTTTGTGTGG	GATCAGTCAGTGGGCGATGG

Images

Construct Design Sequences

sgRNA sequence	TTAAATTCACTCGCAATAAGTGG
sgRNA2 sequence	AGGCAATGTAACACTAGACGCGG

 

left_200bp	right_200bp
AACAATCACTTTATGCATCAAAATAGGTTTATTTTTTAGCAAATAATTAACTTAATATACGCCAATTGCAAACAGAATACCAATTACTTGTAAGCACAAAAAACAGCTGACGGCAACAAGTGGTTCGGTCCCCATCGGAATACACGTGCTCAAAACGTGTGGGTTTTATTTGCCTTAATTGACTTAAATTCACTCGCAAT	ACGCGGCTTGGCAATAAATGAACCTACGTAAATTTGAGTCATTTGTTGTTGTTTTGAATCTCAATCCCACCGTTTTGCTGCTGATGCAAGCGGCTTGAGGAGTATCTGATAACCCTACACCTCGCTAATGGGGACCACAGACCGCAGGGGAGGTCGTTGCCTAGCCAGAAAAGCGAAAACGCGTAAACATGTTTGTGCAC

 

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