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CRIMIC CR70449

Gene ND-B14

CG7712

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:10897144..10897885	47C6	FBti0226931	97324

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGCTGAGCAGCAAATCCTAATCC	GACGAGAGCAAGAAGACTGTCTG

Images

Construct Design Sequences

sgRNA sequence	GCTTGGAGCAAGTTTGTCGTAGG
sgRNA2 sequence	TGAATAACTAATTAACGCCCTGG

 

left_200bp	right_200bp
AAATCCATTACAAATTTGCGGAATATAATGTGGGTATTAAAGATCATAATTTGATATGAGTTTCATTATAGCAATTTAGCAACATCTATTATTTTCGAGCAGAAAACATTTTTAAATAAAAATCATTTTACCAAACTTTGGTTTTGTCAATTTGTGCGTAGTGAACTGCGGTCACACTGTTCTGCTTGGAGCAAGTTTGT	CGTTAATTAGTTATTCAAATTGAAATAGTTTAAATACAAACTCCGACAGAGTGTAAAACGAAACGAAGGGAGTTTTATGCGCTTCAGAAACGCGTGTGTCCTTGTTTATTGAGATTGTGACTTCAAAAATATCATGGTTTCACTGTGAATACATACTAATGAATGTTCTGATCTGGATTCTGAATCGGTTGAGCAAAATA

 

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