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CRIMIC CR70450

Gene Rbpn-5

CG4030

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:21088386..21091004	57B19		98544

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATACATTTGCAAATTGGCGAAGGG	CACAAACTTTATGTTGCATGGCTC

Images

Construct Design Sequences

sgRNA sequence	CCATCAAACAATATAGAAAATGG
sgRNA2 sequence	GATACTACGATTACGATCCGAGG

 

left_200bp	right_200bp
TTTATCAAGCTCAATAAAGTTAGATTTTGAAAACCTTTTTTAACGGCACTGCCAAGCAATACTTAAGTATTAACTATCGGTATTATTCAAATATGGTAACACCAAGAGCAGTGTTGAAAATCGCTCAAATGTATATGTTCTAATTGGTGCGCTCACATATGTACATATAAACTAATAAATTGGCCATCAAACAATATAGA	CCGAGGTCAAACCTTTCCAATGCCCATTTCCATTTGCCAATGTCTATTTCCGATTTTGTTGTTCCATTATTTTGGCCCTATACATAACGTAAGCGATGAGTTGTCTATGTTAGCTTTCGTCGTAGGTAAGTTTTCGTAACTGAATTTAACTCAAAGCAAATATACAAATTAAAAACAATTAAATTTTTGTTGTTTGTCGC

 

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