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CRIMIC CR70451

Gene CG6443

CG6443

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10728978..10730302	32A5	FBti0226932	97325

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATCGTCATTGCAGTCGCTAC	ATGCTTTCTAGAGACGAGGCAG

Images

Construct Design Sequences

sgRNA sequence	TGAGATAAATATATAATCATGGG
sgRNA2 sequence	AATCTAGACTAATTGTAGAATGG

 

left_200bp	right_200bp
CACAATTTCTGCATTTACTACAACCTGAAGATATATAGTTATTTATATCGTTAGTTTATTTCAAGTAAGTAAGATACTTATACCGTGTAACATGTAAAACAATTGAAAAATATCATAATTCATTGTAAATCACATATTTTAGTTTGCCCAACAAGGAATTCCCAGGAAATATATTGTAATTCTTGAGATAAATATATAAT	TACAATTAGTCTAGATTTCGTTTAAGTTTGCTGTGTTAACTCAGTCACATGTCCATAGTTTGATCGTTGAAACCACACACACAGTTTCAAAATATACAACCGACAATGTATCGTGCAGCCTATCAAAAAGGTTTCCTTACCGTGCTTTACAGCGTAGGCAGTTCGCCCCTGAACAACTGGTCTTCCTATGTGAGTATCTG

 

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