CR70452

CRIMIC CR70452

Gene B-H1

CG5529

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:17397478..17403278	16A4-16A5		98481

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGTTAATGGTTCTGTTGTGAAAC	CCAAAAGCGAAGTAAAACGAACC

Images

Construct Design Sequences

sgRNA sequence	GCATGCCCCCAAGTTTGAGGAGG
sgRNA2 sequence

left_200bp	right_200bp
GGAGCGCGGGGGTGGGGCGGCGGCCCTCGTCGATGTCGCTTTGCAAAACAAACTGTAAAAATTATTGCATGACCATTTTACATTCTCGTTCTTGTTGCTTCTATTTGTTGTGGTTTTAGTTGAAAAAAAAAAATACTTTTTCACGCTTTGTTTCCGTCATTGCGAAGCTTCTAAAGTCCCCTTGCCTCCTCGCCCCTCCT	CAAACTTGGGGGCATGCACCTGCGAATCTGAGAGCTATTATGGCAGAATGGAGGCGTGGCTCGCAGTGGGCGGGGGGTTGGCGGAAGAAGAGGATCTGCCTGAAATGCAACAACAAAATGCAGTTTAAAACTGCCGCAGCCGCAGAAAAAATCTCAAATAATTACACACATCGCACTCACTGTTCCCCGCTTCGCCGCTC