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CRIMIC CR70453

Gene B-H2

CG5488

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:17314581..17324175	16A1	FBti0226877	97178

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACAAGCTGGAGCTGAGC	CTGTGCTCGAAATTGTGACAAGTC

Images

Construct Design Sequences

sgRNA sequence	AGCATTCATCACCTTATCGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGAATCGCAGGTGAGCGGCACGCTTTGTGGTTTAATGGTGACTGCAGAGGGGGGTTTTTTACGGGGGCTCTCTGAGTGTTCGGTAATCAGCGCCTTTAATGCGTTGCGCCCATTTCTTCGCGCAGTTTTCCAATAAAATTCCTCTTAACGGCTGCTTTTCCTCTTCTTTTTTTTTGCTCTTCAGCATTCATCACCTTAT	CGTTGGCAGTGTTTTTTTTATGCTGATTGCTTAATTTGCTTCATTTTGCGTTGTGAGTCGTTAAACGAAAAATTCTCTTTGGCAAATGAGGCGTCTGGTTTTTGCGTATTTTTCTCAGTGCAAGTTTGTTGCCTAAGTCTTTCGTTTCGATTGCCGCCTTATTACGTTCGATCGCGTTTTATTTTCGTTTTTAATTATTG

 

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