CR70465

CRIMIC CR70465

Gene ICA69

CG10566

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:21131446..21133310	78B2	FBti0226934	97327

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGATTTCATAAATTATGGAAAATATTTGGATCAAATG	GCTTACGTCGCGTCAAAGAG

Images

Construct Design Sequences

sgRNA sequence	CCTCCTAAACAACCACTCAGTGG
sgRNA2 sequence	TTTGACTAGCTAGTTTCCAACGG

left_200bp	right_200bp
ATTTGGCAATTTCTACTAAATACCATGACTTAACTTAGATCCCTTAAATACCACCCGAATAAAGGACTTTAGTGTGACCCTATACAAACGGGGCGCTTGACAGTGCTGCAAAGTGACGATTGCAATCGAAAGGCGATAAGCATTGGAAAATAACGATACAGCGCCACCCCACCACCCAACCACCCTCCTAAACAACCACT	GAAACTAGCTAGTCAAAATGGAGAATCTTTGGATGTAACTTTTCGCTAAACGTTCAAAAACTTTTTATGTTTTATTTAAATAAAATTTATATGGGACACAGCAGCTAGAAGTGGATGAGGCGGACGAGAGGCAGGTAAAGTGGAGCAGGAGCCGCACTCATTTGACCGCCGCCTGTGCCTCCTTCTTGGAGTTAACCAGC