CR70467

CRIMIC CR70467

Gene ND-30

CG12079

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:3158485..3159578	63B7	FBti0226936	97329

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK?	OK	GTTTTGTGGTTGGTAATGAAGAATAATTGTAAATG	GTTTTCGGATGTGCTAGATCCGG

Images

Construct Design Sequences

sgRNA sequence	ATATTGAACAGTTTGTAGAATGG
sgRNA2 sequence	ACCATTTAACAAACCGACTTCGG

left_200bp	right_200bp
TGTTAACGTACACGAAATACAATAATGTATGTGCGGTATTTGTGTTGTACCGAAATTGTCAAAATACCGAAGTAGGGCTGTCGAGTAGATTAATTTGGCGCACTGACAACCCTAGGCTCCAACAACTATATTGACATTTTAGCATTACATAAATTGTCAAAAACGGAGCAAATTGTTGAATTTATATTGAACAGTTTGTA	TCGGTTTGTTAAATGGTAATTAGATCAATAAAACGTGATCACTATGAGATGTTTAAACATAATGGAAACGACGGTCTACTTAGCTATTTAGCTTTCTTGGCTCGTTCCTAAACTACTTCCACATGAAAACGCAATTCGTATCAAACCAGAGGGTCACAAGTATTTATTGATTTCCGTATTGTTTACATACATGGTAGTTT