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CRIMIC CR70471

Gene apolpp

CG11064

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	4:1060969..1077273	102F7-102F8	FBti0226878	97179

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAAAAAGTCAAAGTTATAAGGATTTGGAATATTC	GTACTTCAAAAGTCCATTGTCAGCTGTG

Images

Construct Design Sequences

sgRNA sequence	CGATTTATAGACCTTATAGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAACCGCAATGTCCAACTGGATTCTTTTTCAGTAAATTGTTAAAAACGGATAGCAAGAGAAATTCTTTTGCCAATAGCTCCGTTAAAAAATCATATATTTCATAAAAATCGTTTGAGTCCCATCAATCAAAACATATAAAATTAGAGGACATTGCACCTTTAAAACTCAAAATCATTGATAACGATTTATAGACCTTAT	AGATGGTTCAGGTAAATACATAAGTGGTAAAGGGGGAACCCAAATTTTAAAATTCTTTCTGGTGATACTAATATTTATTTGGCGACTTGGAGATTACTTTTCCTTAATTCATTCTATACAAACATTTTCTTTTTAATTTTAGAAAACGCTTGCAATCTAGGATGTCCAAAATGTAAGTAATACAAAAAAAGTATGTATTT

 

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