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CRIMIC CR70474

Gene CG15011

CG15011

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:4255991..4259152	64A12	FBti0226903	97332

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAGCAGACTGTAAGTCTTAACG	GATGCCAGTTCCGTCAGTAGAA

Images

Construct Design Sequences

sgRNA sequence	AACAACATTAATATTCTTTCAGG
sgRNA2 sequence	TAGACAAACACTGCGCTGGCTGG

 

left_200bp	right_200bp
AACTTCTAGCCTGCGGCAAGCACAAATGTAATCAGGTGTGTCATCAGCCAGGAAAGTGTCCTCCATGCACCAGCAAAAGCTTACAACCTTGCGAGTGCCAGCGGGAGTCGAAGATGGTCAACTGCTCCGATCGGAAATGGAAGTGTCAGAATGTAAGAAAAGCCTTTTGAAGCTGAAGCATAAAACAACATTAATATTCT	AGCGCAGTGTTTGTCTATGTTGAGCTTTTCTAATCAGGCTGGCCAATTACTCCGCCTTGGCCTTCTTCTTGTAGACGCAAGCGGCGGTGAAAACGGAGGCGCTAAGGAACGTCGAAATCAGGAAGTTTGATAGCAACATCCAATCGTGGGACTGGAAGAACACCGTGTAGATTCGAGCTGTAAGGGATACATATATGATG

 

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