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CRIMIC CR70475

Gene CG31997

CG31997

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	4:154654..156060	102A4	FBti0226880	97181

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTAAAAATGAGTTTTCATTTTGCTGTACTGACC	GTATTTCTTAACAATTTCAATTAATTTTCGGGCTG

Images

Construct Design Sequences

sgRNA sequence	AAACGGCATGTTGTTAAATCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTATTTTAACAGCCTTCACAGTTTCTCTGTGTGCTGAACAAAAAATTACAAAGAGTGACGCAGGTGAAATACGAATTTTCAAACGTCTTATTCCTGCCGATGTTCTACGAGGTAAGTATGGCAATCATCAGATTTAGAAATTTTCCATTATTAAAAGTTACAAGTTCAATATAAGTATATCTAAAACGGCATGTTGTTAA	ATCGGGTGACACGCGTATAGTTTTAAGTAACATAAAAGGTATGGGCTAGTGTAACGCAAAAAAAAAACAACAACTAAATATCCCTCTCCTTTCTCAAGGTATTAATTTTGGCCACAAAAGGTATCATTCAGCCTATGGTGAACATTTATCGAGTGTTTTTGCTTTTGATGTATACGTGATCTATTATATAGTTTCCACAG

 

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