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CRIMIC CR70476

Gene CG32006

CG32006

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SA_kG4	4:150764..153538	102A4	FBti0226965	97333

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK?	OK	TTTGTGAGTTCAGCAATAAATAGGTG	GATTGTAATGTAACATTCTGTTTTGCCG

Images

Construct Design Sequences

sgRNA sequence	GCGGGCGGACATGACGAAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTGAAAGTTTATGTGATTCATATATCTAACTATATATTTTTAAAAATGTTCATTTTCCATTGTGTATAAAAATAAATATAATGCATTTTGTGCAAGAGTATAAAATTTGAATACCTTGCCTAACAAGTGTTGCCATATTTAGTTTTCAACTTGATTTGTTAAAAAATTTGATTGATTAAGCTAACCAGCCACACCGATT	TCGTCATGTCCGCCCGCCCGCACGACGGATCTCCTGAACTACAGATACATACATATGTATATACAAATAAGAAATAAATTTATTGTGTAGTACTTTAAAATAATAATTGATTTAAATTAACGCTCTGAAGTCATAGAAATAGCCTTTTTGGGCTCTTGACTAATATATATATAATAACTGAATGAACTAAATTATTTTTG

 

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