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CRIMIC CR70477

Gene CG33941

CG33941

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	4:467497..471476	102C1	FBti0226881	97182

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAACCATTGAACTCGAGCATTG	TGCGCACTGAAGTGTATTCGTG

Images

Construct Design Sequences

sgRNA sequence	TGAAATCTTCAGTATATGTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAAAACATTTTTTCATAGAGAGCGGGATCTTAGGACCCGTTAACTTTAAATATTCTCATGACATGATTCGGAAGACGTCTACCATTCACATTGACGAAACTCTTAAGAATCAGGTAAGGGTGGATAATCTGATTCCCAACGAATACACGTTTACACCTATGTTAACCATAATAGGTGTATAGGTATCGTATTTCCTTAC	ATATACTGAAGATTTCATATGCAGTCGGTCAGTTATCGAGTAACTGGTTAGTCCGTCTGACTATCAATCTGAACGCTGTGATCTCGGGAACAATGAAAACTAGAATATTGATATTAAGCATTAAGACTCAGTCACAGAGTCTGTAAGAGTTTGTTTCATGCCGTTGCGACGCTTGCTCCAACGACGACGCACCCTCAATA

 

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