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CRIMIC CR70478

Gene CG33978

CG33978

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	4:157575..175639	102A6		98482

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGAGAACGGCAGTTTGTTG	AGAGATTGACCAAAATCAAAGTGATTAAGTAG

Images

Construct Design Sequences

sgRNA sequence	ACGGTAATCGAATTAAGGATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAATAATTAAAAAAATATATTTATTATACTTACAGATCAGTCTTATCATGTGAGTCCGACACCAACTTTGCCGGCAATATCCGGGGATGGTAAGAAAATTAAAACAAAGCGCTGTTGAATTAGTTACAGCTTAATATTTTATTTAAATATTATAATTGTGTTCTTATTTTACGTATTTTAAAACGGTAATCGAATTAAG	GATTGGTTCACAATAAAATTTTTTATAACTATATAGTATTTTATAAATTAAAATATATTCTTATTCATTTTCTTTATCTCTTTCTATGATTTCACCAACTTTTGGAATGTACATTCGGAAACAATAACTTACTTTATCTTTTATCTTACGCCTGCACAAAATGACATTGTCCTGTTTCTTCTATGTGACTTTCACGTTTT

 

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