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CRIMIC CR70479

Gene Crk

CG1587

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	4:210224..213165	102A8	FBti0226882	97183

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATTTTGTGTGTTTCAGTTTTGGGAAAC	GTAAACGATTTGATCCTGTTGTTGAACTTTG

Images

Construct Design Sequences

sgRNA sequence	TATGTGCGCCATGTATTGACGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCTTTCCCATTCAACTAGCTGAGTAACGGGTTCTCTCTTGTTTTGTTTGATATTTAGATCCAATGGCAAGGGTCTATGGTCAATTTTGTATTTCTTTAATTTAGTATGGAATGTACCTTTAAGGACGATGATAAAATGTCAATGATGATCTCTCATAGCCATTGCAATTTAGTATGTTTTTAATATGTGCGCCATGTATT	GACGGGGTAAATAAATTGAAGAATTTAACGACGTAAACAAAAATAATTACGAATTAAAGGAACTCAATTTCTACTGTATGTCTCGCCACGCGGCATTTATTAAAGAATTAGATGAATAAACGTCTCAAGAATATTCTTTTTGATTTTAAAAGTTCTTTATTAATATAAATGTATATACATTAGAATATTAAAAATAGGTT

 

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