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CRIMIC CR70482

Gene gw

CG31992

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	4:649041..660608	102D2-102D3	FBti0226883	97184

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGCACACAAATTCTTAATTAGCG	CACTCCACAGAGTTGAATCCACTC

Images

Construct Design Sequences

sgRNA sequence	TATTCGTCTCGTTTGACTCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTCCAACAGGTGGCACATTTGGCAGTAATTTTAATAGAAAGTTCTATGTACTCAACTATAATATGAAAGTTTGTTTAAAATATTTTGTGAATCAATCTACTGAATGCTTTTTTGTTTGTTTGTTTGTTGCGACATTTGCGCGAATTGGTATGTTGGTTTTATCGTAGGTTACCGTAGTTTGGTATTCGTCTCGTTTGAC	TCTTGGACATTTTAAGACAATATAATATAACCGTGAAGATTTAGAAAGTGCGGGTCCTAGCAACGCTTACGAGCGTGGTTCATGCTGTAAGCATAGCATTTGTAATTCATTTACATTTGTTAATTTATATTTATATTTATACCTTTTACTCGCTTAGTCAAAGGGTATAGTATATTATTAGAAAAGTAAGTTGCGGGGTA

 

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