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CRIMIC CR70483

Gene Jyalpha

CG45760

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	4:879..5039	101F1	FBti0226884	97185

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAACCCAGCACGAACCAC	CTCGTATAACTGTAGCGTATTGTGGAAC

Images

Construct Design Sequences

sgRNA sequence	GTTATTATTCTCTAGAGTAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGACAATCTCTATCTTGGCATTGCATTGACCGTTTTGGTAATAGTCACTGGATTATTTACTTATTTTCAGGTAAATAAATGAATATGTCAAATAAATATTTCTAAATATTACAATTATCCTTATTGAAGTTAAGTCTAAGTTTGATTTTGTTCCTTTTTTTGATATTCAGAGATCCGTTTCTGTTATTATTCTCTAGAG	TAAGGGTATACTGGATTCGTCGATAATTTTTTTCTCATTTTATTTCCCAATATCTATCGATATCCCAGAAAAAATTATGAAATTTCGCATTTCCACTAGTTGAGTAACGGATATCTGACAGTCGGGGAACTCGACTGTTGTATTCTCTCTTGTTGTAATACAAAAATTCTTTAGTATACAAATAATTTTTAACATTATTG

 

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