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CRIMIC CR70484

Gene PIP4K

CG17471

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	4:1192419..1196848	102F8	FBti0226885	97186

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CCAATAGTACCAATATAGTATACCAATTTACC	AAAGAGTAATCCATAATGTGCAACTTGG

Images

Construct Design Sequences

sgRNA sequence	GGGAAGCTGTAAGTGCTATAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTGAATTGCCTTAACGTTGTTTTTATTTAAATATAGCTTAGAAATAATAATAGCCTAATCTTCTATGTACATAATACCAGAAAATAAATTTCAAAAATGACTTTATATTAGAATATTTGTCATTAGAGTATTCAGCTTGCGACGACTAGACTTGTTGAATCGATCTGAAACATCGAAATTTGGGAAGCTGTAAGTGCT	ATAAGGAGTGCAATTAgCATATCGAGGATCATCGTCTTACATATCTTGAATGTTATTAATTCATAGGGACTACAAATTTGTCGCCTTGCACATTTTATATAAATTTCAGGTCTTGAAAATCATACGAATGTGCTGCCATTTTCAAGCATATTGCGTTAAATCTAATTCATAAAAAAAAGTCCTACGAAGTGGTACTTGAA

 

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