CR70486

CRIMIC CR70486

Gene Zip102B

CG2177

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	4:310930..314087	102B3-102B3	FBti0226904	97334

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGATTGAGTTGGCAAAAACATG	ACTACCATTCTGAGAACGTAAACTATCC

Images

Construct Design Sequences

sgRNA sequence	TACTTAATAAATGTCATTAAAGG
sgRNA2 sequence	TCACAATACATTCTCGTATAAGG

left_200bp	right_200bp
CATGGCTGAGGAGACAATAATTCTCATTTTATTAGTGTTGGTTATGCTTGTGGGGTCTTATTTGGCAGGGAGCATACCGATGCTGATGAAATTAAGCGAGGTCGGTACTAAACAATGGCATTTTAAATACAAAAAAAATTTAATGTGAGGTATTTGGAGCACCACTAGTGCATTAATTTGTTCTACTTAATAAATGTCAT	ACGAGAATGTATTGTGAGTAAACCAAGCAAAATGAGGAAGGTCAAAAAGTAGTGCAAGAAATATTTTACTTTAATATTTAGACTTGCGCAGGCAGGAATAGTACGCAAGTCGGCGCCGAATTCATTGGCATCAGAATTTTGACTCCGGCTCCTATATACTATATGTTAATTTATAATTATATATGTTTTTATTGTATTTA