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CRIMIC CR70488

Gene Hel25E

CG7269

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:5539326..5542314	25E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	no band	GGTCACACTGCTCTGCG	CTTCTCTTCCTGTCGCAATGC

Images

Construct Design Sequences

sgRNA sequence	TATAGCCGATAACTAGTTAAAGG
sgRNA2 sequence	AAACGGAAATGTGAAACAAGTGG

 

left_200bp	right_200bp
TCGTCGCTTGTAATAGGTATTTCGTTTTATTTCGAAATACATGTAATGCGGTGCGGTGAAAAAACAAAATGGCGGAGGGAACGCCGGCACCGGTTCCGCAAAAAAAAGCGGCATTTTCTTTAACAAACACATGTAACTTATCTAGTCGCACCCATTTTATTTCCCAGCATTTAGTTGCGAATTTATAGCCGATAACTAGT	GTTTCACATTTCCGTTTAAACTATTTTATTTGTGAAAATTTCTGGGGCATATTCCCTTAAATAACTTAGCAATATTAATGTGTACAATATTATAAATCAACTCACAATAAAATGCCCAAAAAAAATAACAAACTCGCATATGCTTTAATGGTGAAATGGTAATTGGGAACTCGCTGGGCATAGTTTAGTGTGTCCGTTTC

 

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