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CRIMIC CR70490

Gene CG15035

CG15035

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:7401698..7403174	7B2	FBti0226939	97335

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		GTGAGATTCGATTGGAAAAGCAAAATAG	GTTCGATAACTGGCATAGTTGTAGGC

Images

Construct Design Sequences

sgRNA sequence	ATAAAGGAAAGCGCAGGCTATGG
sgRNA2 sequence	CAGTGTCGTCTAGAATAGATCGG

 

left_200bp	right_200bp
AACAATACACTTTATGTTAGGTGGAATTTTATTTTTAACAACTGATGGATGATAGATGGCGTTGATTTAGTCGAAATATATGTCAAATAGATGTGACACCCCTTCGATTTAGTTTTCGTCTGACATTCGTTTGAATAGCACACAATCGCGTAGATTTCGTATAACATATACACATAGTTTTGTATAAAGGAAAGCGCAGG	GATCGGATATCCAAGGCTATATACAATCATTGAACAGCCACTTCGTAGCAAATTTTACTGACAAAAAAGAATTGTTCCAAAAAACTTTTCAATCACTTATCACTTAACACAATCACTTATAGTAGATATTCAATCATTTAAGACTCTTTTCTTTTCAACTCTGCCATCACAAACCAATTTGTTTTGTACCTCTTTTTGTT

 

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