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CRIMIC CR70491

Gene Gapvd1

CG1657

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:11325385..11334071	10B3	FBti0226940	97336

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAAAGCGAATGCGAATGG	TCAGCTTCAGCATCGGCATC

Images

Construct Design Sequences

sgRNA sequence	GCGAAGGCTGCACCTAGCCATGG
sgRNA2 sequence	GCGATATTTGTTAAGGGCTTAGG

 

left_200bp	right_200bp
TGTGTAAAAACATACGGAAATAGCTGGAATTATAAATAAAAATATATTTGCGAAACTGGATAAACAATGGTCGTACTCTTCCGTACGGAAATTAATGCATAATATACATAATTCCATTTAATTCAAAGCACATCTATAACTCAATTCACATTAATTAACATTTTGCTGCTTTTATTCCAGCCAGCGAAGGCTGCACCTAG	CTTAGGCGGATGTATCTGTAGATTTGTTTATCGCTTTGTTGTAGGCATTGCACCAATCTCCCATCCCACTCTCTCCTCCAGTCGATATTTTCCATATTTATATATGTATATGTATGTCTGTGTCTGATATTTGTGTAAAAATTTGTTAAAACCATAGTCTAAGTGCAAAAACTGCGGCAGATGTGCGAAGCGATCCAAGT

 

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