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CRIMIC CR70493

Gene Mask

CG33106

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:24230562..24250248	95F3-95F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	CAGCGTGGACTATCTGCTGC	GCACCTCACCCTCGTGTG

Images

Construct Design Sequences

sgRNA sequence	CAACATAATCCATTAACTGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAAAAGAGTCTTCTCTCTGTGCCTGCGTTAATTTTTATTATTTATGTACACGTTTCTTTCGTTTCTCTTTATTTGTAGATCCTATTGGCATTCTTGTGTTTAAGGCCAGATCTCCTGGTAAGTTTCAGTTTCGCGTTATAAATCAGTTTCCCGATCCGCATCCCAAAAGTTAAAAGAAAACCCAACATAATCCATTAAC	TGAAGGCAAAAGGCAAACGAAAGTACCTAAACCTTCACACCACCAGTTGTACTAAAACATTAATTTACGCAGAATACTTGATCGATCTATAACCCAATTACTCCGCTTCCTCCTCGCACACATGATTAAACACACCTAAACCACACCACTTTGTTGCTGGCCTTAGACAACAAGAATGGGCTCTACTGATCAACTATTAA

 

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