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CRIMIC CR70494

Gene Htt

CG9995

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:28696242..28734816	98E2	FBti0226888	97189

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCAATGTTTTCCTGTGTTG	GCGGCATAGTTGATGTGGC

Images

Construct Design Sequences

sgRNA sequence	TATGAAGCCCAGGTGATTTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGCGAAAATGGACAAATCCAGGTCCAGTGCGTACGACAAGTTCGTTGGCTTCGTGGAACAACTGCGAAATACGGAATGCAGGTGGGACTGAACGGAAATTTGATTGTTTTCGGTTGCATTGAATGTTTTGGGGAGGGGACCGGCAACAGTTTGTTGCTCATTACGGAACAATATTGATTAAATTATGAAGCCCAGGTGAT	TTCCGGTGGTTTTATTGCCCTTAAGATGAACCTGCCAACTTGTTAGGCCACCCAAAACATTCATTCAACATTGAGCAATAAGTGCATCCGCTATGACGTCATGCCACATACCTGTAATAAAACTAATTTATTTCGAACCCCCCACAGCCAAAAGCAGAAGATCACCTGCTTTCAGCAGATTGCCGAGTGCATCATGTCGC

 

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