CR70495

CRIMIC CR70495

Gene AP-2a

CG4260

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:408030..414777	21C2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGTTTGTGGACAACATATAGGAGAG	CTACTGCCTCGAAATGATGCAAC

Images

Construct Design Sequences

sgRNA sequence	ATATTCACCCATTTGCCCACAGG
sgRNA2 sequence	CACCCCGTCCATGATTGTTGTGG

left_200bp	right_200bp
ACTAATTGACGCCTATGTGAATCGGAGAAAATCCGCGACATGGAGAGAGCTGAAGGGTGTGAAGTCGTCTCTCCGATCGATCAATCGCGGGGTGGAAAGGAAAGCGAAGCTTCCGCAGACGAATCACTGTTGTTTTACTGTGAATCTCCCTTATACCCGCATAAGACACAAATTTCTATACTAATATTCACCCATTTGCC	CAATCATGGACGGGGTGATGGAGACGGAGCTGCGAAGCCATGGAGCAACGGCAACCACTTTACAATGCACAATCCCAATTACCTAGAGAAATATCAAAGCATACAAGGCCCTAGAACTGTACGCGTTTAGTTATGCCAGGCACACTCCTACACTCTTCTTAAGTACCTTTCATCTTCTCCAACAGCGAGTTGATTTACCC