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CRIMIC CR70496

Gene Sk2

CG32484

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:3047087..3050159	63A3-63A4	FBti0226889	97190

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTTCTTGAGCACCAGGTG	CGGGAAGTTGAGCTATAGATGAAC

Images

Construct Design Sequences

sgRNA sequence	TAAACTGCATGGCTAGACGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GACTGCTGCAGCGCCAGGACTGGGCCCACGTCCTGCCTCATCTGGCACTGGGAATCATTCCTTGCGGCTCCGGAAATGGATTGGCCCGCTCCATTGCCCATTGTTACAAGTATGTTATCTTTGTTTCATGTTTCATTAGGAAAAACCCCCTTTTGACTTATGTGGATCTTCATATTTCGTGTAAAACAATCCCTCCAGCG	TCTAGCCATGCAGTTTATCATTAGATTACATTCATAAGCAAAAGTAGTTTTCTTATCGTGTCAAATGGTTTTGTTGTTTTGCAAGTGGGGATATCCCCTTAAAGTTAAATTGACGTCAGACTAGAACAAACTTTCCACATCGTTAAGTAATTGCCCCCCAATTTCGTTCAACATTTAGTGGCATATCATCGAACTTTGAA

 

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