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CRIMIC CR70501

Gene san

CG12352

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:11361331..11361332	47F5	FBti0228267	97728

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCCTACTACAACGACATTGTAG	GAACTCGATGGCTCCGTTG

Images

Construct Design Sequences

sgRNA sequence	ATCGAACTACATTGCTCCCCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTCGCATCGACAACACTGAGAACCAGCGGCGCCTGTATATCATGACCCTAGGATGCCTCTCCCCGTACCGGCGCCTGGGCATCGGCACAGTTATGTTCGAGCACATTATGAACTTCGCCGAGAAGGACGGAAACTTCGACAGCATTTTTCTGTAAGAAAACTTATCATTAAGATTCGTGTGTTTTCGACACACCCCTGGG	GAGCAATGTAGTTCGATTTTAATGTAACCTATAACTATTTAAGTGGGAAAGTTTAATTCCGGCCTATCTGGCTTATTTATCTATCCATTTAATACATAATATATATATTTTTGGCTAAGGAGGAAATAGGTTTATTTCGAAAATACTTTAGATCATGTTTTTTAATTCTGGTTATAATTTTAGACATGTGCAAATCAACA

 

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