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CRIMIC CR70502

Gene Naa60

CG18177

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:9726895..9729650	67C5		98485

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGCTTAACCTGTACAGGCG	AGGAACCTTAGCTGCACATCG

Images

Construct Design Sequences

sgRNA sequence	AAACAAAAGCGGCCCATTGACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGACAGAGCAGCAGCAGACGCAGTGGCAGTGGCAGTGGGAACGGAGGAGCACCAGCACCAGCACCATCAGCAGCAGCAGCACCCCCCCTTGCAATAGGATGGCACAATTCACACTGTGAGTATACGTACATATAACTATTTGGTTATCAGTCGGGCTGCACACGGATTTCCTGCTTATGCGGACACCGGAGCCCCGTCA	ATGGGCCGCTTTTGTTTATCTATTGACGGCGCTTCGGCGGTATTATGCAAGGGAACTCAATGTTTCGCCGCCTTTTGGCTTGACTAATACATGAGGATCATGAAACATTATCATACCATTGCGAGCATTGCAGCTACAACAAACACAGTGCGCCGCCCAGTAGCGACAGTACGCGTGTGGACTGCGAACACGTACCGTTG

 

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