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CRIMIC CR70504

Gene ass

CG1315

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:6642262..6644434	84A1	FBti0226891	97192

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCCTACGCCCTGAAACC	CTATTTGTCGGTTGAAATGGGGC

Images

Construct Design Sequences

sgRNA sequence	CGTAACGATCGAGTAGGTTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTCGGGTTGGATTGCCTCTCTTATCAGCCCCTTATCGCACGCCAAGTCAACAACAAAGTTGTGCGTACCCAAGTTGTCTGACTAGCTACATTAACATCTTTGAGTGTAATCTATAAACAAATCAATGCTACAAGAAGTGCCGTGAACGCATAAAATTTTAGCTTTTGGTTTGTGGTTTAAACCGTAACGATCGAGTAGG	TTCCGGTTTAAGAACCGGTTCGTCGACATGCTGATCAAATCCATTTCAAGTTACGGCTTAAATCCGTTTTGCTTGTTACTTTGAAATCACAAAGATTGCACTATTTTTTAGTTTTTCTTCATTTCAAATAAAGAAGTCAAGAGCTTTTTGGTTTGCCATGGTTTTTGTTGCTTAGCAGGCAGAATATTTCATCACAATGA

 

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