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CRIMIC CR70505

Gene CG7857

CG7857

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:15503600..15504894	71D3	FBti0226942	97338

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTGATGCGTAACAATGAATGGAG	GAATTTACACTAAACCGCCACAAG

Images

Construct Design Sequences

sgRNA sequence	CGGTATATAATAACGCAAGCTGG
sgRNA2 sequence	CCTAAACGGGAAATGCCCAATGG

 

left_200bp	right_200bp
GATTTTTGTTTAAATTTTCCATCCCAAATGATATAACAAGAAATAAATTAGTTTATTTAAAGATTATTCATTATATTACAGAAATTTCAAATTCAAACTTGGTCTTTTTCCATACAGTCAAAATACTAAATACTAAAGTAAAATAAAGTATATTATAACGGTGACGATATTTCCGACAGCTTTCGGTATATAATAACGCA	CAATGGCATTTACCATCAACTCACTCGATTTTGTAACAGATTGTTTTATTACATATTATGTTGACTTTATAAATACGTATGCATTTGGAAGATTTGAATTATTGTCAAAAATTGCAAGTATGTAGTAGTTATATACAATAATAGTCAATATAATAACAATTCTAGGTTAGCTAGCAACTTAGTTAGCTGGATTTGTTCTA

 

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