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CRIMIC CR70507

Gene Sec16

CG32654

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:12596047..12609910	11B2-11B3		98486

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCATCTAGAAATACCCCAGAGTCTTG	GACGGAGTGGTGGTGTAGATG

Images

Construct Design Sequences

sgRNA sequence	AGTTTCGGGCTCTAGAATTTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTGGAAAAAAAAGGCAATTTTTTGCTTGATATTTTTATACTATTCGTTCACTGGAGCGTAATAAGTATCTTTCGCTTAGTGTAAAAAAAGATTATATAAATTAGATGGCATGTTGTTATTTAACTATTCAAATTAAATTTATTTCGTGTAATGAAATTAAGTAGGACAGTGTAATCTCTGCAAGTTTCGGGCTCTAGAA	TTTCGGCTGATCCAAAGTGTTCCTTTGCCAACGTTCCTTTAGTTTTGTTTAACCCCTGTCACCTGATACCTTAACCCTTAGTCTGAACCCGTTTACCTGCTCCTAACTTTTTGAACTCGAAGTAGTTGTTGGTTTTTCTCTGGCTTTCTTACATTACCTTGATGTTTGTCAACTAAAAACGGATGATTAACATTGATTGT

 

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