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CRIMIC CR70514

Gene Rtnl1

CG33113

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:4992808..5009720	25B9-25C1		98487

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGACATTTTCATATACGTTCTGCCCC	GATAAGGGATTCCACTGGAAAGAAGAG

Images

Construct Design Sequences

sgRNA sequence	AACTGTGGCACCATCTGCCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGGCAGCGAACGTCAAACGTAGGAGCACCGCGAACGATCCGTTGGAAAACCCGAAAACTTGAATACCCGAAAAATGTCGAACAGATTGTGTGAGTGAACCAACCGGGCTGAGAAAAGGGGGTGCTTGGGTTCAGATATTATAGTATCAGAGCTTAGTTGCATGCCGTTGAACAAATGCTAACGATAATTACCACCTCGG	CAGATGGTGCCACAGTTCTCGAATCTGTATCTCATCTATTTATCTCATAGATTGCATAATTGCCGCCAGCTGAAAAGATATATGTACATAGATGCGTGCGAGACGCTGAACGTATGTGTGCCTCGTATATTTGGGGATTCTCAGTCCACATTTTCCACTTGTATCTCGTCTCTTTTCACAGTTTATGAGGGTTATAATTT

 

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