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CRIMIC CR70515

Gene Rala

CG2849

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:3704610..3719635	3E5-3E6	FBti0226892	97193

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTATTTCAACTCCAATTGAATGCCCC	CTGTTCACACAACGGATTGGC

Images

Construct Design Sequences

sgRNA sequence	GGCAGAGGACAGGGTGCGACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTGCCTTTAGTATGCCATATCATGTACTAGCGATAATTACCGACCGATGAATATGCTACTTCGTTGCCATAACTCCCACTTAAGGCGCCCCCTTTTCCATCGACCTAATTGAGTATTCAAACTTGGCAACTTAATATTGCTGCTGCCACATAAATATGGCTAGAAAATTGTTATTAGACGTCGGCAGAGGACAGGGTGC	GACTGGCCTGGGAAATACTTATGTATATCCAGTATATTCGATCCCTACTATTTATGCGTTCGCCTGTCCACTGTCAGTGTGTTCACATAAATATATATCTATATATCTGCTTGACTGCTAGGTTTTTCTCTGGCTTCTAAATGGAGTGACACACGAACTGAGTCACTTTTATATTGAAGTGCTGATAAGTACGCTTATCT

 

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